Table: Migration of bromophenol blue and xylene cyanol on agarose gel in TBE and TAE electrophoresis buffer

Position of bromophenol blue and xylene cyanol in agarose gel in relation to the position of double standard DNA fragment in TAE (1x) and TBE (0.5 x) electrophoresis buffer.
For example in 0.5% agarose gel, Bromophenol blue migrates at approximately 750 bp long double standard DNA fragment in TBE buffer and at approximately 1150 bp long double standard DNA fragment in TAE buffer.
Agarose gel, % Bromophenol blue in TBE buffer (0.5x) Xylene cyanol FF) in TBE buffer (0.5x) Bromophenol blue in TAE buffer (1x) Xylene cyanol FF in TAE buffer (1x)
0.5 750 13000 1150 16700
0.6 540 8820 850 11600
0.7 410 6400 660 8500
0.8 320 4830 530 6500
0.9 260 3770 440 5140
1.0 220 3030 370 4160
1.2 160 2070 275 2890
1.5 110 1300 190 1840
2.0 65 710 120 1040
Reference:
  • https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0012614_Gen_Recommend_DNA_Electrophoresis_UG.pdf