Agarose gel

  • Agarose gel can be used for the separation of nucleic acids (DNA and RNA) and high molecular weight proteins or protein complexes.
  • The process of using agarose gel to separate or fractionate biomolecules under the influence of electric field is called agarose gel electrophoresis.
  • For moderate and large sized DNA, agarose gel is the most commonly used medium in most molecular biology laboratories.
  • As the name suggest, the agarose gel is prepared from agarose.
  • Chemically, agarose is a polysaccharide, made up of alternating residues of 1,3-linked β-D-galactopyranose and 1,4-linked 3,6-anhydro-α- L-galactopyranose.