- Kanamycin is a water-soluble, aminoglycoside bactericidal antibiotic.
- It was originally purified from the bacterium Streptomyces kanamyceticus.
- It inhibits protein synthesis in prokaryotes by binding to the 30S subunit of the bacterial ribosomes.
- Kanamycin is effective against a wide variety of gram-negative and some gram-positive bacteria.
- Deionized / Milli-Q water
- Equipment and disposables
- Measuring cylinder
- Conical flask / Beaker / 15 ml screw-capped tube
- Magnetic stirrer
- 50 mg/ml kanamycin
- Preparation of 10 ml of 50 mg/ml kanamycin stock solution in water
Step 1: To prepare 10 ml of stock solution of kanamycin, weigh out 500 mg of kanamycin. Transfer it to 15 ml screw-capped tube. Add 9 ml of deionized / Milli-Q water. Mix until all kanamycin is completely dissolved.
- We recommend to use 15 ml screw-capped tube with millilitre marks. Transfer all the contents in it. Add 9 ml deionized / Milli-Q water and mix the content by rotating the tube. Once the content is mixed, make up the volume 10 ml.
- Do not dissolve in 10 ml of deionized / Milli-Q water. In most cases, solution volume increases when the large amount of solute dissolves in solvent.
- Use autoclaved deionized / Milli-Q water.
Step 2: Adjust the volume to 10 ml with deionized / Milli-Q water. Mix it again.
- Store the solution in small aliquots (1 ml) at -20°C for long time.
- A selection marker for bacterial culture in gene cloning experiments.
|Follow the table to prepare kanamycin solution of different concentration and volume.|
|Conc. / Volume||5 ml||10 ml||25 ml||100 ml|
|50 mg / ml||0.25 g||0.5 g||2.5 g||5 g|
|100 mg / ml||0.5 g||1 g||5 g||10 g|