Preparation of stock solution of Kanamycin


  • Kanamycin is a water-soluble, aminoglycoside bactericidal antibiotic.
  • It was originally purified from the bacterium Streptomyces kanamyceticus.
  • It inhibits protein synthesis in prokaryotes by binding to the 30S subunit of the bacterial ribosomes.
  • Kanamycin is effective against a wide variety of gram-negative and some gram-positive bacteria.


  • Reagents
    • Kanamycin
    • Deionized / Milli-Q water
  • Equipment and disposables
    • Measuring cylinder
    • Conical flask / Beaker / 15 ml screw-capped tube
    • Magnetic stirrer


  • 50 mg/ml kanamycin


  • Preparation of 10 ml of 50 mg/ml kanamycin stock solution in water


Step 1: To prepare 10 ml of stock solution of kanamycin, weigh out 500 mg of kanamycin. Transfer it to 15 ml screw-capped tube. Add 9 ml of deionized / Milli-Q water. Mix until all kanamycin is completely dissolved.

  • We recommend to use 15 ml screw-capped tube with millilitre marks. Transfer all the contents in it. Add 9 ml deionized / Milli-Q water and mix the content by rotating the tube. Once the content is mixed, make up the volume 10 ml.
  • Do not dissolve in 10 ml of deionized / Milli-Q water. In most cases, solution volume increases when the large amount of solute dissolves in solvent.
  • Use autoclaved deionized / Milli-Q water.

Step 2: Adjust the volume to 10 ml with deionized / Milli-Q water. Mix it again.


  • Store the solution in small aliquots (1 ml) at -20°C for long time.


  • A selection marker for bacterial culture in gene cloning experiments.
Follow the table to prepare kanamycin solution of different concentration and volume.
Conc. / Volume 5 ml 10 ml 25 ml 100 ml
50 mg / ml 0.25 g 0.5 g 2.5 g 5 g
100 mg / ml 0.5 g 1 g 5 g 10 g