- Chloramphenicol is a broad spectrum antibiotics which is effective against both gram positive and gram negative bacteria.
- It is bacteriostatic agent.
- It inhibits bacterial protein synthesis by irreversibly binding to 50S ribosomal subunit and inhibiting peptidyl transferase catalytic function.
- 70% ethanol
- Equipment and disposables
- Measuring cylinder
- Conical flask / Beaker / 15 ml screw-capped tube
- Magnetic stirrer
- 34 mg/ml chloramphenicol
- 70% ethanol
Preparation of 10 ml of chloramphenicol stock solution in 70% ethanol
Step 1: To prepare 10 ml of stock solution of chloramphenicol, weigh out 340 mg of chloramphenicol. Transfer it to 15 ml screw-capped tube. Add 9 ml of 70% ethanol. Mix until all chloramphenicol is dissolved completely.
- We recommend to use 15 ml screw-capped tube with millilitre marks. Transfer all the contents in it. Add 9 ml 70% ethanol and mix the content by rotating the tube. Once the content is mixed, make up the volume 10 ml.
- Do not dissolve in 10 ml of deionized / Milli-Q water. In most cases, solution volume increases when the large amount of solute dissolves in solvent.
Step 2: Adjust the volume to 10 ml with d70% ethanol. Mix it again.
Store the solution in small aliquots (1 ml) at -20°C for long time.
- It is recommended to store the chloramphenicol stock solution in small aliquots.
A selection marker for bacterial culture during gene cloning experiments.
|Follow the table to prepare chloramphenicol solution of various volume (5 ml, 10 ml, 25 ml, 100).|
|Reagents / Volume||5 ml||10 ml||25 ml||100 ml|
|Chloramphenicol||170 mg||340 mg||850 mg||3400 mg|
|70% Ethanol||Adjust the final volume to 5 ml||Adjust the final volume to 10 ml||Adjust the final volume to 25 ml||Adjust the final volume to 100 ml|