- Sodium dodecyl sulfate (SDS), also known as Sodium lauryl sulfate (SLS), is an anionic surfactant (detergent).
- It is extensively used in biological research as a strong protein denaturant and lipid-solubilizing agent.
- It is also used in our cleaning and personal care products, e.g., shampoos, hand soaps, bath products, shaving creams, toothpaste.
- SDS is water soluble, white to yellowish powder.
- Heating dissolves SDS quickly in water, thus helpful to prepare concentrated SDS aqueous solution (20% SDS solution in water). Once dissolved, SDS forms clear transparent or slight yellowish solution.
- 10% or 20% (w/v) stock solution of Sodium dodecyl sulfate (SDS) is generally prepared in distilled water (or milli Q water).
- Sodium dodecyl sulfate (CH3(CH2)11OSO3Na, Molecular weight: 288.38, CAS Number: 151-21-3)
- Deionized / Milli-Q water
- Equipment and disposables
- Measuring cylinder
- Conical flask / Beaker
- Magnetic stirrer with heat control (optional)
- 10 % Sodium dodecyl sulfate
- Preparation of 100 ml of 10 % SDS in Deionized / Milli-Q water.
Step 1: To prepare 100 ml of 10 % SDS solution, weigh out 10 grams SDS in 250 ml conical flask / beaker. Add 80 ml deionized / Milli-Q water and mix it.
- One can use manual shaking using a glass pipette to mix the ingredients. Magnetic stirrer makes the dissolving process easy and convenient.
- Do not dissolve in 100 ml of deionized / Milli-Q water. In most cases, solution volume increases when the large amount of solute is dissolved in solvent.
- Wear face mask to avoid inhalation of SDS dust.
- Try to avoid frothing.
Step 2: Heat to 68 °C if necessary.
- One can set the magnetic stirrer temperature 68 °C. If magnetic stirrer is unavailable, place the solution in a water bath set at 68 °C.
- Make sure to dissolve any residual powder sticking to the glass.
Step 3: Adjust the volume to 100 ml with deionized / Milli-Q water. Mix it again.
Step 4 (Optional) : One can filter the solution to remove any undissolved material.
- Do not autoclave 10% SDS solution.
- Solution can be stored at room temperature for several months.
- Do not store the solution at 4°C. SDS will precipitate at temperature below 15°C. If there is any precipitate, redissolve by warming the solution (68°C for 10 min)
- Plasmid isolation by alkaline lysis method
- Preparation of Laemmli sample buffer
- Stripping of immunoblot
|Follow the table to prepare Sodium dodecyl sulfate (SDS) solution of various volume (10 ml, 100 ml, 500 ml and 1,000 ml) and percentage (1%, 5%, 10% and 20%).
|Reagents / Volume||10 ml||100 ml||500 ml||1000 ml|
|1%||0.1 g||1.00 g||5.00 g||10.00 g|
|5%||0.50 g||5.00 g||25.00 g||50.00 g|
|10%||1.00 g||10.00 g||50.00 g||100.00 g|
|20%||2.00 g||20.00 g||100.00 g||