- DTT is used to stabilize enzymes and other proteins which possess free sulfhydryl groups.
- Formula : C4H10O2S2
- Molecular weight : 154.25
- DL-Dithiothreitol (C4H10O2S2) (Molecular weight = 154.25)
- Deionized / Milli-Q water
- Equipments and disposables
- Measuring cylinder
- Conical flask / Beaker
- Magnetic stirrer
- 1 M Dithiothreitol
- Preparation of 25 ml of 1 M Dithiothreitol solution
Step 1: To prepare 25 ml aqueous solution of 1M DTT, weigh out 3.86 gram of DTT (Molecular weight = 154.25). Transfer it to 50 ml beaker/conical flask. Add 20 ml deionized / Milli-Q water. Mix until all DTT is dissolved completely.
- One can use manual shaking using a glass pipette to mix the ingredients. Magnetic stirrer makes the dissolving process easy and convenient.
- Do not dissolve in 25 ml of deionized / Milli-Q water. In most cases, solution volume increases when the large amount of solute dissolves in solvent. see solution preparation
Step 2: Adjust the volume to 25 ml with deionized / Milli-Q water. Mix it again.
Step 3 (Optional): One can sterilize the solution by passing through 0.22 μ filter unit under aseptic condition (inside laminar flow hood).
- Do not autoclave the solution. Autoclaving will destroy the DTT.
Solution should be stored at -20°C.
- It is recommended to store the solutions as small aliquots.
- Avoid repeated handling and multiple freeze/thaw cycles.
- Enzyme stabilization
- Enzymatic reactions
- Protein electrophoresis
|To prepare Rubidium chloride solution of different concentration and volume, follow the table.|
|Conc. / Volume||5 ml||10 ml||25 ml||50 ml||100 ml||200 ml|
|10 mM||0.0077 g||0.015 g||0.0386 g||0.077 g||0.154 g||0.309 g|
|100 mM||0.077 g||0.154 g||0.386 g||0.77 g||1.54 g||3.085 g|
|0.5 M||0.385 g||0.77 g||1.93 g||3.86 g||7.71 g||15.43 g|
|1 M||0.77 g||1.54 g||3.86 g||7.71 g||15.43 g||30.85 g|